On the other hand, because proteins and other biomolecules would rapidly bind to the nanomaterial surface, it is very difficult to predict the biological behaviors of nanoparticles in physiological environments. In fact, Prussian blue staining was developed and has widely been accepted for analyzing the distribution of iron oxide nanoparticles within different tissues. The specificity and sensitivity are the foremost requirements for any chromogenic reaction being used for histochemical analysis. The chromogenic reactions were typically performed at pH 1.
The colorimetric responses of Chlorophosphonazo III to other RE ions were also characterized by absorbance spectroscopy. It is noteworthy that among the hosts for upconversion emission, fluoride materials e. After that, each cell sample was divided into two portions.
The slices obtained were then divided into two groups. The first group of slices was stained with Chlorophosphonazo III after the adsorbed particles were eroded with strong acid and the slices without particle contamination served as reference. The color of the slices changes from purple to blue followed by gradually thickened green with the increase of particle concentration, well in consistence with the in vitro cell staining results.
As shown in the top graph, the plot of G vs R can be partitioned into four different regions according to the G and R values. Therefore, the R, G, B values were set in the range of 0—, —, and 0—, respectively, for further quantitatively analyzing the staining results given in the left column. Apparently, the background i. The tissues of liver, spleen, solid tumors were harvested 6 h post injection the corresponding tissues of mice that received no injection of nanoprobe were also collected and used as control.
In fact, liver and spleen as the major organs of reticuloendothelial system RES are responsible for purifying the blood of foreign particles.
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Shanghai, China. Other chemicals of analytical grade including hydrochloric acid, nitric acid, ethanol, and dimethylbenzene were purchased from Beijing Chemical Works and used as received. Porcine muscle tissue was purchased from supermarket. Some other wells did not do any processing to serve as control.
SERS tags: novel optical nanoprobes for bioanalysis.
The resulting cells were incubated with the staining solution for 15 min, washed with ultrapure water, and then subjected to microscopy study. After deparaffinizing, the slices were immersed in the staining solution for 15 min at room temperature, washed with ultrapure water for three times, and then sealed with neutral balsam. The histochemical studies were carried out when the tumor size reached 5 mm.
At 6 h post injection, mice were sacrificed. Tissues of liver, spleen, and solid tumors were collected, embedded into paraffin, sliced, and then stained with Chlorophosphonazo III. All animal experiments were performed according to a protocol approved by the Peking University Institutional Animal Care and Use Committee. Optical Density Calculation : The slices after Chlorophosphonazo III staining were all scanned at the same parameters and the images were exported through software CaseViewer without any processing.
Then area and IOD were then obtained using the Measure command. Nanomaterials for Fuel Cell Catalysis. Kenneth I. Organic Optoelectronic Materials. Yongfang Li. Thomas Baumgartner. Fullerens, Graphenes and Nanotubes.
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